Figure 2. Cdc14 phosphatase inhibits rRNA transcription and prevents binding of Pol I subunits to ribosomal genes.
a, Primary rRNA transcript levels in metaphase-arrested cells with expression of wild-type CDC14 or phosphatase-dead CDC14-C/S. b, Primary rRNA transcript levels upon expression of a stabilized form of cyclinB2 (CLB2-DK) in the presence of a wild-type CDC14 or temperature-sensitive mutant cdc14-1. c, Localization of Rpa43 and the nucleolar marker Cfi1 in CLB2-DK-expressing cells with wild-type CDC14 or cdc14-1. Scale bar 5 µm. d, ChIP analysis of Rpa43 binding to 35S DNA in cells before (left) and after (right) expression of CLB2-DK in the presence of a wild-type CDC14 or cdc14-1. An average (n = 2) and s.d. are shown.