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. 2014 Oct 13;5(5):114. doi: 10.1186/scrt504

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© Brennan et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Characterization of bone marrow stromal cells. (a) Representative results of phenotypic characterization of good manufacturing practice-grade bone marrow stromal cells (BMSCs) by flow cytometry. More than 90% of BMSCs express CD73, CD90, CD105 and HLA A-B-C, and <5% of cells had surface antigen expression for CD3, CD34, CD45 and HLA DP, DQ, DR. (b) Tri-lineage differentiation capacity was confirmed. Adipogenic (Oil Red O/hematoxylin staining), chondrogenic (Alcian blue staining) and osteogenic differentiation (Alizarin red staining) could be induced, whereas no differentiation could be observed in controls without induced of differentiation. Black scale bars: 250 μm for adipogenic, 500 μm for osteogenic differentiation, and 1,000 μm for chondrogenic.