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. 2015 May 28;16:177. doi: 10.1186/s12859-015-0616-y

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© Sanders et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Synapse clump splitting. a Original image with green signal being GABAergic synapse. b Enhanced image with the yellow arrow indicating staining artifacts (dim green signal) and the red arrow indicates synapse clump. c synapse pre-detection (randomly color-coded). d Synapse centers detected using discriminative model; e model-guided splitting to split a clump into two synapses (randomly color-coded). f Example of synapses after iterative model-guided splitting (randomly color-coded). Visualization is done by Vaa3D