Table 2.
Results of transfucosylation reactions catalyzed by α-l-fucosidase iso2 from P. thiaminolyticus
| Acceptor | Transglycosylation products | Acceptor | Transglycosylation products |
|---|---|---|---|
| pNPα-L-Fuc | + | methanol | +/* |
| pNPα-D-Gal | + | ethanol | +/* |
| pNPα-D-Glc | + | 1-propanol | - |
| pNPα-D-Man | + | 2-propanol | - |
| D-glucose | + | butanol | - |
| D-galactose | + | pentanol | - |
| D-fructose | + | 1-octanol | - |
| D-mannose | + | Boc-L-Ser-OMe | - |
| D-glucosamine | + | Boc-L-Thr-OMe | - |
| N-acetyl-D-glucosamine | +/* | ||
| D-maltose | + |
pNPα-l-Fuc served as a donor of α-l-fucosyl moiety in all cases
The symbol + indicates that the enzyme was able to use the acceptor for α- l -fucosyl transfer
The symbol - indicates that no transglycosylation product was detected by TLC
The symbol * indicates that the transglycosylation product was confirmed by mass spectrometry