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. 2015 May 27;6:244. doi: 10.3389/fimmu.2015.00244

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Copyright © 2015 Tobar, Arancibia, Torres, Vera, Soto, Carrasco, Alvarado, Neira, Arcos and Tobar.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Chromatography of purified IgM from S. salar. The IgM of Atlantic salmon previously separated by SEC was subjected to HPLC on dC18 column (Waters) and eluted under isocratic conditions. The chromatography was monitored at 280 nm.