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. Author manuscript; available in PMC: 2015 May 27.
Published in final edited form as: Cancer Res. 2008 Jul 1;68(13):5226–5235. doi: 10.1158/0008-5472.CAN-07-5744

Figure 1.

Figure 1

Breast cancer cell lines have variable levels of CBF-1 transcriptional activity; estrogen up-regulates Notch-1 but decreases Notch-4 expression. A, Western blots showing expression of Notch-1 and Notch-4 in a panel of breast cancer cell lines as well as HMEC controls. GAPDH, glyceraldehyde-3-phosphate dehydrogenase. B, CBF-1 luciferase activities of the breast cancer cell lines shown in A and HMEC controls. Cells were transfected simultaneously (n = 3) at comparable levels of confluence and tested side by side. Data are normalized for efficiency of transfection using a Renilla luciferase plasmid. Columns, mean; bars, SD. Data are representative of at least three independent experiments. RLU, relative luciferase unit. C and D, Western blots. C, 10–9 mol/L 17β-estradiol (E2) in steroid-free medium for 72 h up-regulated Notch-1 in T47D:A18 but not T47D:C42 cells. Results represent five independent experiments. D, 10–9 mol/L 17β-estradiol in steroid-free medium for 72 h up-regulated Notch-1 but decreased Notch-4 in either MCF-7 or T47D:A18. 4-OH-TAM (1 μmol/L) antagonized both effects. Results represent three independent experiments. EtOH, ethanol.