Figure 2. Ruthenium red sensitivity and single-channel properties of mPiezo1 and dPiezo chimeras.

(a) Schematic representations illustrating portions of the protein sequence from mPiezo1 (black) or dPiezo (red) for each construct. (b) Representative (from five to eight experimental replicates) MA current traces at −80 mV from cells transfected with constructs shown in a before, during or after application of 30 μM RR. Each trace is an average of —three to five trials. Probe stimulation displacements are indicated. (c) Percent inhibition of MA currents in cells transfected with specified constructs in the presence of 30 μM RR (n=8, 5, 6, 5 and 5; mean±s.e.m.). One-way analysis of variance (ANOVA) with Dunn's comparison with mPiezo1 or dPiezo, *P<0.05, **P<0.01. (d) Representative (from five to seven experimental replicates) stretch-activated channel openings elicited at −180 mV from cells transfected with specified constructs. dPiezo and mP1^1–1,973/dP^{1,930–2,548} traces are displayed after applying 1 kHz digital filter. (e) Average I–V relationships of stretch-activated single channels in cells transfected with specified constructs. Single-channel amplitude was determined as the amplitude difference in Gaussian fits of full-trace histograms. (f) Unitary conductance of stretch-activated channels from cells transfected with specified constructs. Conductance is calculated from the slope of linear regression line of individual cell single-channel I–V relationships (n=7, 5, 5, 5 and 6; mean±s.e.m.). One-way ANOVA with Dunn's comparison with mPiezo1 or dPiezo, *P<0.05, **P<0.01.