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Real-time PCR for (a) genes involved in the ubiquitin-proteasome system (UPS), (b) the autophagic/lysosomal pathway, and (c) Transforming Growth Factor-β (TGF-β) signaling. (d) FoxO3 gene expression of obese myotubes expressed as fold relative to lean. (e) FoxO protein levels. (f) Representative blots of FoxO3 and membrane stained for total protein to demonstrate equal loading. *Significant effect versus basal (P < 0.05). †Significant main effect (obese versus lean, P < 0.05).
