A — Concordance correlation coefficients (CCC) between optical density ratios (ODR), calculated at readings from 1.5 to 15 min, and the endpoint-ODRs (eODR), calculated at 28 min for a total of 27 plates. B — Similarly, CCC were calculated between slope ratios (SR) for readings from 1.5 to 15 min, and eODR at 28 min, for a total of 27 plates. Arrows are placed at 5.25 and 10.5 min. The CCC show the level of agreement between the various reduced processing times, calculated as either ODR or SR, and the conventional protocol for eODR measured at 28 min. Locally weighted scatterplot smoothing curves (black lines) show the trending patterns for the CCC over time.