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. 2015 May 27;2:15019. doi: 10.1038/mtm.2015.19

Figure 2.

Figure 2

Confocal fluorescence microscopy images of whole-mounts of the auditory epithelium (second turn) lesioned with an ototoxic insult and preinjected with Ad.HSP70-mCherry showing expression of mCherry (a,e,i), HSP70 (b,f,j), localization of phalloidin (c,g,k), and merged (d,h,l). a–dxy images are at focal plane of Deiters cells, e–hxy and i–lxy are the same fields at a lower plane. mCherry expression is seen in Deiters cells (a) and pillar cells (e,i), corresponding to cells expressing HSP70 (b,f,j). c, g, and k show nonsensory cells labeled for phalloidin (blue) for facilitating orientation in the tissue. Merged images (d,h,l) confirm fusion proteins HSP70 and mCherry colocalization and show absence of HSP70 in cells negative for mCherry. dyz, hyz, and lyz are orthogonal sections showing Deiters cell or pillar cell (*) adjacent to the IHC (arrowheads). DC, Deiters cell; P, pillar cell; HSP, heat shock protein; IHC, inner hair cell. Bar = 20 μm.