Table 1.
Semen parameters for the first evaluation and during the daily ejaculation period of 2 weeks. (Mean ± SEM, n = 6)
| First | Day 2 | Day 4 | Day 6 | Day 9 | Day 11 | Day 13 | |
|---|---|---|---|---|---|---|---|
| Abstinence, days | 3-4 | 1 | 1 | 1 | 1 | 1 | 1 |
| Volume, mL | 2.5 ± 0.2 | 1.8 ± 0.4* | 1.8 ± 0.3* | 1.6 ± 0.2* | 1.9 ± 0.3* | 1.7 ± 0.4 * | 1.9 ± 0.2* |
| Sperm concentration, 106/mL | 121.3 ± 35.5 | 80.3 ± 18.7 | 72.1 ± 18.1 | 66.1 ± 15.2 | 75.1 ± 22.6 | 80.4 ± 21.5 | 80.3 ± 19.2 |
| Total sperm count, ×106 | 276.8 ± 70.5 | 134.4 ± 46.6* | 115.7 ± 27.8* | 88.7 ± 16.2* | 111.5 ± 23.6* | 109.9 ± 27.3* | 138.2 ± 31.4* |
| Total motility, % | 53.83 ± 4.7 | 55.50 ± 10.2 | 54.0 ± 7.8 | 52.9 ± 7.0 | 58.5 ± 5.7 | 58.7 ± 4.4 | 51.70 ± 5.5 |
| Progressive motility, % | 47.0 ± 5.5 | 51 ± 11.3 | 43.0 ± 8.6 | 45.2 ± 8.0 | 46.6 ± 6.4 | 50.5 ± 5.0 | 40.8 ± 3.3 |
| Vitality, % | 60.2 ± 3.1 | 61.7 ± 8.8 | 53.4 ± 7.4 | 65.5 ± 5.3 | 60.2 ± 4.7 | 59.5 ± 3.1 | 69.8 ± 5.6* |
| Morphology, normal forms % | 13.8 ± 8.2 | 12.1 ± 9.3 | 10.3 ± 7.6 | 11.5 ± 6.8 | 10.4 ± 9.4 | 12.9 ± 9.1 | 10.2 ± 6.9 |
| ROS production, MFI | 84.8 ± 9.3 | 58.5 ± 1.6 | 64.3 ± 3.8 | 79.7 ± 13.7 | 71.8 ± 18.6 | 63.3 ± 15.4 | 42.6 ± 6.1 |
| Mitochondrial Membrane Potential, % | 49.4 ± 8.1 | 49.8 ± 12.1 | 43.3 ± 9.2 | 49.5 ± 9.7 | 48.0 ± 6.0 | 47.7 ± 7.2 | 51.5 ± 8.6 |
| DNA fragmentation index, % | 25.6 ± 3.0 | 22.1 ± 4.3 | 24.0 ± 3.4 | 27.1 ± 3.5 | 24.7 ± 2.8 | 25.8 ± 4.6 | 23.9 ± 4.7 |
| Membrane integrity, % | 59. 9 ± 7.4 | 51.2 ± 7.5 | 53.0 ± 5.9 | 63.0 ± 5.8 | 56.7 ± 6.9 | 58.7 ± 4.4 | 57.8 ± 6.8 |
MFI Mean Fluorescence Intensity, MMP Mitochondrial Membrane Potential, ROS reactive oxygen species
*p < 0.05 vs. first analysis