Table 5.
Pathways for downregulated genes after expansion in serum-free medium (top 10)
| Pathway | Gene symbol | Count |
|---|---|---|
| Cell cycle | BUB1; BUB1B; CCNA2; CCNB1; CCNB2; CCNE2; CDC20; CDC23; CDC25A; CDC25C; CDC45L; CDC6; CDC2; CDK2; CDKN2C; CHEK1; ESPL1; MAD2L1; MCM2; MCM7; MCM3; MCM4; MCM5; MCM7; ORC1L; PLK1; PTTG1; SKP2; SMAD3; TFDP1; TGFB1; TGFB2; WEE1; YWHAH; YWHAZ | 34 |
| Cytokine–cytokine receptor interaction | CCL2; CCL26; CXCL1; CXCL3; CXCL5; CXCL6; TNFSF13B; EGF; EGFR; FLT1; IL11; IL1A; IL1B; IL6; IL7R; IL8; INHBA; LIF; PDGFC; PF4V1; CCL14; TGFB1; TGFB2; TNFRSF11B; VEGFA | 25 |
| Focal adhesion | ACTB; COL4A6; COL6A1; EGF; EGFR; FLNB; FLT1; GRLF1; ITGA4; ITGA5; ITGA6; ITGB3; MAPK8; MYLK; PAK2; PDGFC; PDPK1; PRKCA; PTK2; TLN1; TNC; VASP; VEGFA | 23 |
| Regulation of actin cytoskeleton | ACTB; BDKRB1; DIAPH3; EGF; EGFR; FGF1; FGF2; FGF13; GRLF1; IQGAP3; ITGA4; ITGA5; ITGA6; ITGB3; MYH9; MYLK; NRAS; PAK2; PDGFC; PIP5K1A; PTK2 | 21 |
| Mitogen-activated protein kinase signaling pathway | DUSP4; EGF; EGFR; FGF1; FGF2; FGF13; FLNB; IL1A; IL1B; MAPK8; MAPKAPK2; NRAS; PAK2; PRKACB; PRKCA; RPS6KA3; MAP3K7IP2; TGFB1; TGFB2 | 19 |
| DNA polymerase | FEN1; MCM2; MCM7; MCM3; MCM4; MCM5; MCM7; POLA1; POLA2; POLE2; PRIM1; PRIM2; RFC2; RFC3; RFC4; RFC5; RNASEH2A | 16 |
| Cell adhesion molecules | CD276; CDH2; CLDN1; ITGA4; ITGA6; JAM3; NEGR1; NEO1; NRXN3; PDCD1LG2; PTPRF; PVR; PVRL2; SDC1; VCAN | 15 |
| Gap junction | CDC2; EGF; EGFR; GNAS; NRAS; PDGFC; PLCB1; PRKACB; PRKCA; TUBA4A; TUBB2A; TUBB; TUBB2C; TUBB6 | 14 |
| Pyrimidine metabolism | CTPS; POLA1; POLA2; POLE2; POLR2D; POLR3G; PRIM1; PRIM2; RRM1; RRM2; TK1; TYMS; UCK2 | 13 |
| Purine metabolism | GART; PDE7B; PDE8A; PFAS; POLA1; POLA2; POLE2; POLR2D; POLR3G; PRIM1; PRIM2; RRM1; RRM2 | 13 |