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. 2014 Dec 2;23(2):244–254. doi: 10.1038/mt.2014.206

Figure 2.

Figure 2

Lentiviral vector validation in vitro. (a) Transduction with Lenti.CDNF-GFP induces CDNF overexpression in HEK293T cells; (b) Transduction with Lenti.MANF-GFP induces MANF overexpression. Scale bars = 100 µm. Immunoblotting showing; (c) increased CDNF expression in culture supernatants and cell pellets with higher Lenti.CDNF-GFP MOI; (d) Increased MANF expression in cell pellets transduced with Lenti.MANF-GFP (MOI 5); (e) Increased GDNF expression with higher Lenti.GDNF-GFP MOI. α-tubulin was used as an endogenous loading control; (f) Immunocytochemistry on transduced E18 rat cortical neurons (MOI 5); left, control virus Lenti.GFP; middle, transduction with Lenti.CDNF-GFP virus stained for CDNF (red) and GFP (green); right, transduction with Lenti.MANF-GFP virus stained for MANF (red) and GFP (green). All sections were counterstained with Hoescht to label cell nuclei (blue). Scale bars = 50 µm.