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Processing of CDNF-T2A-MANF. (a) Translation of the CDNF-T2A-MANF gene as one single polypeptide; (b) removal of signal peptides (SP) and T2A-induced self-cleaving yields four peptidic fragments, including CDNF+19 aminoacids and native MANF; (c) increased intracellular CDNF and MANF as well as secreted CDNF with higher Lenti.CDNF-T2A-MANF-GFP MOI (+ = positive control).
