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. 2014 Nov 25;23(2):387–395. doi: 10.1038/mt.2014.207

Figure 1.

Figure 1

HXTC manufacturing process. Peripheral blood mononuclear cells (PBMCs) are isolated from 60–100 ml of whole blood samples from aviremic HIV+ patients. Monocytes are separated using plastic adherence and used to generate dendritic cells. Dendritic cells are pulsed with PepMixes spanning Gag, Pol, and Nef before being used to stimulate the nonadherent PBMC fraction which contains T cells. T cells undergo two more weekly stimulations by coculture with autologous, PepMix-pulsed PHA-blasts. During the third stimulation, irradiated K562 cells that have been genetically modified to express costimulatory molecules CD80, CD83, CD86, and 41-BBL are cocultured with the T cells in addition to the PepMix-pulsed PHA-blasts.