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. 2014 Dec 9;23(2):330–338. doi: 10.1038/mt.2014.219

Figure 4.

Figure 4

Growth and cytotoxic activity of NK-92/5.28.z cells upon γ-irradiation. (a) To investigate the effect on viability, NK-92/5.28.z cells were irradiated with 5 or 10 Gy and cultured for up to 8 days. Proliferation (left panel) and percentage of viable cells (right panel) were analyzed by counting viable cells at the indicated time points using trypan blue exclusion. Mean values ± SEM are shown; n = 3. (b) Cytotoxic activity of NK-92/5.28.z cells 24 hours after irradiation with 10 Gy against ErbB2-positive MDA-MB453 and ErbB2-negative MDA-MB468 breast carcinoma cells was determined in FACS-based cytotoxicity assays at different effector to target ratios (E/T) as indicated (filled circles). Parental NK-92 cells 24 hours after irradiation were included for comparison (open circles). Mean values ± SEM are shown; n = 3.