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SUMO-2/3 localizes to the mitotic chromosome protein scaffold and centromeres. Mitotic chromosome spreads were labeled using DAPI and stained with SUMO-2/3 antibodies and either (A) CREST, (B) phospho-histone 3 or (C) Smc4 antibodies. Chromosomes were analyzed by immunofluorescence microscopy. Bar = 1 μm.
