Figure 1. Effects of chronic exposure of C2C12 skeletal muscle cells to silibinin or palmitate on glucose uptake. Myotubes were incubated for 16 h in growth medium supplemented with 100 μg/mL silibinin or 0.75 mM palmitate, and then left untreated or stimulated with 100 nM insulin, followed by assessment of 2-NBDG uptake as described in Methods. Data are reported as means±SD of 4 determinations. *P<0.05, compared to control basal values; ^#P<0.05, compared to control group treated with acute insulin (Student's t-test).