Figure 2. Effect of silibinin on insulin-stimulated glucose uptake in palmitate-treated C2C12 myotubes. C2C12 myotubes were incubated with palmitate (0.75 mM) or silibinin + palmitate, then stimulated with insulin (100 nM), followed by assay for 2-NBDG uptake as described in Methods. Data are reported as means±SD of 4 determinations. *P<0.05, compared to control basal values; **P<0.05, compared to insulin control values; #P<0.05, compared to palmitate group (Student's t-test).
