Figure 4. Effects of silibinin on the palmitate-inhibited insulin signaling pathway in C2C12 myotubes. C2C12 myotubes were incubated with palmitate (0.75 mM) or silibinin + palmitate as described in Methods. Before harvesting, the cells were incubated in the presence or absence of 100 nM insulin for 30 min and lysed for Western blotting. Data are reported as means±SD of 4 determinations. *P<0.05, compared to control group; **P<0.05, compared to insulin group; ^#P<0.05, compared to palmitate group (Student's t-test).