Figure 6. Silibinin modulated the phosphorylation of IRS-1 Ser307, JNK, IKKβ but not PKC-θ in skeletal muscle cells. Differentiated C2C12 skeletal muscle cells were incubated with palmitate (0.75 mM) or silibinin (100 μg/mL) + palmitate, then stimulated with insulin (100 nM) before cells were harvested for Western blotting. Data are reported as means±SD of 4 determinations. *P<0.05, compared to insulin group; ^#P<0.05, compared to palmitate group in the presence of insulin (Student's t-test).