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. Author manuscript; available in PMC: 2015 May 27.
Published in final edited form as: J Biol Chem. 2007 Aug 17;282(41):29882–29889. doi: 10.1074/jbc.M702097200

Figure 4.

Figure 4

In vitro analysis of the amelogenin promoter. A, the C/EBP site is required for maintaining the basal amelogenin promoter activity and C/EBPδ-mediated transactivation. Various reporter constructs (p2207-luc, p70-luc, mC/EBP-p2207-luc, and mC/EBP-p70-luc) each used at 250 ng were transiently transfected into LS8 cells with 200 ng of C/EBPα expression plasmid or empty vector pcDNA3. In all cases, pCMV-lacZ (75 ng) was included as an internal control for transfection efficiency. The relative luciferase activity was the normalization of luciferase activity with β-galactosidase activity. The mean ± S.D. from at least three independent experiments was represented, and the level of p70-luc in the absence of exogenous C/EBPδ was set arbitrarily as “1”. B, C/EBPδ and NF-Y synergize on the minimal amelogenin promoter. LS8 cells were transiently transfected with 250 ng of p70-luc reporter construct in the presence of 200 ng of empty “expression” vector (lane 1), C/EBPδ (lane 2), NF-Y (lane 3), dominant negative form of NF-Y, NF-YAm 29 (lane 4), C/EBPδ and NF-Y (lane 5), and C/EBPδ and NF-YAm 29 (lane 6). pCMV-lacZ plasmid (75 ng) was included in all experiment groups as an internal control for transfection efficiency. Data reflected the mean ± S.D. of three independent experiments, with the response level of p70-luc in the absence of exogenous C/EBPδ set arbitrarily as “1”.