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. Author manuscript; available in PMC: 2015 May 27.
Published in final edited form as: J Biol Chem. 2007 Aug 17;282(41):29882–29889. doi: 10.1074/jbc.M702097200

Figure 5.

Figure 5

EMSA of the C/EBPδ binding site. Nuclear extracts were prepared from LS8 cells transfected with the C/EBPδ expression plasmid (NE/δ). Extracts were incubated with 32P-labeled probe, followed by electrophoretic separation, and visualized by autoradiography. For competition assay (lane 3), 50-fold molar excesses of unlabeled probe were incubated with 32P-labeled probe. For supershift assay, extracts were pre-incubated with a C/EBPδ antibody (lane 4, sc-636x, Santa Cruz Biotechnology, Santa Cruz, CA), normal rabbit serum (lane 5), NF-YA antibody (lane 6, sc-636x, Santa Cruz Biotechnology, Santa Cruz, CA), and normal goat serum (lane 7). The supershifted band is denoted by the symbol “*”.