ChIP analysis for the binding of C/EBPα or C/EBPδ on the mouse amelogenin promoter. LS8 cells (100 mm plate) were transfected with p2207-luc (500 ng) with the expression vector for either C/EBPα (800 ng) or C/EBPδ (800 ng), and incubated for 24 h. Cells were fixed, sonicated and incubated with an non-immune IgG, or an anti-C/EBPα antibody (sc-61x, Santa Cruz Biotechnology, Santa Cruz, CA) or an anti-C/EBPδ antibody (sc-636x, Santa Cruz Biotechnology, Santa Cruz, CA), and immunoprecipitated with protein G-agarose beads (16-201C, Upstate Biotechnology, Temecula, CA).