NF-Y facilitates C/EBPα or C/EBPδ and synergistically induces the endogenous amelogenin promoter as measured by mRNA transcripts. LS8 cells were transiently transfected with 200 ng of empty vector (lane 1), expression vector for C/EBPα (lane 2), C/EBPδ (lane 3), NF-Y (lane 4), C/EBPα and NF-Y (lane 5), C/EBPδ and NF-Y (lane 6). The mRNA expression level from the endogenous amelogenin gene was determined by real-time PCR analysis. Data represents three independent experiments, each done in triplicates. The level of amelogenin mRNA from cells treated with the empty vector was set arbitrarily as “1”.