Figure 3. Impaired CD4⁺ T cells response in MyD88^T-KO mice is caused by defective IL-1 signaling.

(A, B) Expression of the receptors for IL-1 (A) and IL-18 (B) in CD4⁺ T cells in the draining lymph nodes of immunized MyD88^T-KO and wild-type control mice as measured by qPCR. The expression levels were normalized to the level of naïve CD4⁺ T cells. A representative experiment using the pooled samples from 5–10 mice per genotype is shown. (C, D) CD4⁺ T cell response of Rag2-deficient mice reconstituted with a 3:1 mix of bone marrow from TCRβ-deficient mice and IL1R^KO, IL18^KO, or TLR2^KO; TLR4^KO mice, respectively. CD4⁺ T cells were restimulated with OVA in the presence of irradiated splenocytes 7 days after the immunzation of the bone marrow chimeras in the feet with OVA + LPS in IFA. The proliferation was measured 3 days later by ³H-thymidine incorporation (C) and the secretion of IFNγ and IL-17 of the restimulated CD4⁺ T cells was measured by ELISA (D). A epresentative experiment is shown. (E, F). CD4⁺ T cell response of mixed bone marrow chimeras harboring bone marrow of IL1R^KO, IL18^KO, or wild-type mice mixed with bone marrow of TCRβ^KO mice in a ratio of 1:3 after footpad immunization with OVA + LPS in IFA. A representative experiment is shown.