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. 2015 May 20;5:10277. doi: 10.1038/srep10277

Figure 4. Effects of SIRT1 knockdown by shRNA on cell apoptosis and p38 MAPK signaling in cultured PMVECs challenged with burn serum.

Figure 4

PMVECs were exposed to 10% sham serum (SS), 10% SS + non-targeting shRNA (NTsR), 10% SS + SIRT1 shRNA (SsR), 10% burn serum (BS), 10% BS + NTsR, 10% BS + SsR for 12 h. (A) Representative immunoblots showing the protein level change of SIRT1 in each treatment group. (B) Flow cytometry analysis showing the percentage of apoptotic cells in each treatment group. (C) Representative immunoblots showing the protein level change of cleaved caspase-3 in each treatment group. (D) Representative immunoblots showing the protein level changes of p-p38 in each treatment group. Results represent mean ± SEM of six independent experiments using different batches of PMVECs from different rats. *p < 0.05; **p < 0.01, compared to the value in 10% BS + NTsR group.