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. Author manuscript; available in PMC: 2015 May 27.
Published in final edited form as: J Nat Sci. 2015 Apr 1;1(4):e84.

Figure 2. AAH expression in N-Myc-AAH and C-HA-AAH transfected cells.

Figure 2

Huh7 cells were transfected with recombinant plasmid DNA carrying the full-length human AAH cDNA (CMV promoter) fused in-frame with either an N-terminal Myc tag (N-Myc-AAH) or C-terminal HA tag (C-HA-AAH). 24 hours after transfection, cultures stimulated with IGF-1 (10 ng/ml) for 0–60 min were subjected to Western blot analysis using antibodies to (A) Myc or FB50+A85G6, or (C) HA or A85G6. Blots were stripped and re-probed with antibodies to p85-PI3K (loading control). (B, D) Digital imaging was used to quantify the Western blot signals. Graphs depict calculated mean ± S.E.M. signal intensity ratios of AAH/p85-PI3K. Data were analyzed by 1-way ANOVA with the Dunnett post-hoc significance test (*P<0.05, **P<0.01, ***P<0,001; ****P<0.0001 relative to control (0 time point).