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. Author manuscript; available in PMC: 2015 May 27.
Published in final edited form as: J Nat Sci. 2015 Apr 1;1(4):e84.

Figure 4. Co-localization of C-HA-AAH and Notch-1 intracellular domain.

Figure 4

Huh7 cells transfected with C-HA-AAH (A–J) were stimulated with IGF-1. Cytocentrifuge preparations of the cells were double-immunostained with antibodies to (A–D) HA and (F–I) Notch-1 intracellular domain (NID). Immunoreactivity was detected with biotinylated secondary antibody and Streptavidin-conjugated Dylight 547 for HA (green) or Dylight 647 for NID (red). Cells were imaged by confocal microscopy. (K–N) Merged images depict co-localization of HA and NID. (E, J) Negative control studies showing results with primary antibodies omitted. (600× original magnification, 2× digital zoom).