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. Author manuscript; available in PMC: 2015 May 27.
Published in final edited form as: J Nat Sci. 2015 Apr 1;1(4):e84.

Figure 5. Kinase inhibitor modulation of AAH expression.

Figure 5

Huh7 96-well cultures were pre-treated with vehicle, LiCl (GSK-3β inhibitor), TBCA (CK2 inhibitor), Gö6983 (pan-PKC inhibitor), H-89 (PKA inhibitor), or PD98059 (MEK-1 inhibitor) for 4 hrs and then stimulated with nothing or of IGF-1 for 15 min. Immunoreactivity to (A) AAH-A85G6 and (B) β-Actin was measured by cellular ELISA with results normalized to Hoechst H33342 fluorescence (cell density). Graphs depict the mean ± S.E.M of 4 replicate cultures per group. Inter-group comparisons were made by two-way ANOVA (Table 1) and post hoc Fisher’s Least Significance Difference tests (*P<0.05; **P<0.01; ***P<0.001; and ****P<0.0001).