Figure 7. Kinase inhibitor modulation of IGF-1 stimulated shifts in AAH intra-cellular immunoreactivity.

Huh7 chamber slide cultures were pre-treated for 4 h with vehicle, LiCl, H-89, TBB, or D4476, and then stimulated with IGF-1 for 5 to 60 min. Cells were stained to detect AAH using the FB50 antibody, biotinylated secondary antibody, and Cy-Dye fluorophore (red). Cells were counterstained with DAPI (blue) and imaged by confocal laser microscopy. Merged images are depicted.