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. Author manuscript; available in PMC: 2015 May 27.
Published in final edited form as: J Nat Sci. 2015 Apr 1;1(4):e84.

Figure 8. Effects of GSK-3β inhibition on AAH expression after long-term insulin stimulation.

Figure 8

Huh7 cells were transfected with N-Myc-AAH or C-HA-AAH cDNA, pre-treated with vehicle or LiCl, and insulin stimulated for 16 h. (A, B) Western blots were probed with antibodies to Myc or HA, then stripped and re-probed with the A85G6 or FB50 antibodies to AAH, followed by a third probing with anti-p85-PI3K. (C, D) N-Myc-AAH transfected cells cyto-centrifuged onto glass slides, were stained by immunofluorescence with anti-Myc (red) and counterstained with DAPI (blue). Images were acquired by confocal microscopy.