Table 1.

Differential effects of kinase inhibitors on short-term IGF-1 stimulated AAH immunoreactivity

	IGF1 Stimulation		Kinase Inhibitor		IGF-1 × Inhibitor
Protein	F-Ratio	P-Value	F-Ratio	P-Value	F-Ratio	P-Value
A85G6	13.99	0.0003	35.18	<0.0001	18.72	0.0042
A85E6	12.41	<0.0001	59.28	<0.0001	14.88	<0.0001
FB50	3.57	N.S.	57.67	<0.0001	3.18	N.S.
β-Actin	1.40	N.S.	66.43	<0.0001	18.44	<0.0001

Huh7 human hepatoma cells grown in 96-well micro-cultures were pre-treated with vehicle (control), LiCl, TBCA, Gö6983, H-89, or PD98059 to inhibit GSK-3β, CK2, pan-PKC, PKA, or MEK, and then stimulated for 15 minutes with 10 ng/ml IGF-1. Cellular ELISAs measured immunoreactivity to AAH (A85G6-C-terminus; A85E6 and FB50-N-terminus) and β-actin. Data were analyzed by Two-way ANOVA. Corresponding graphs with post-hoc Fisher LSD results are shown in Figure 5.