Table 2.

Differential effects of kinase inhibitors on long-term IGF-1 stimulated AAH immunoreactivity

	IGF-1 Stimulation		Kinase Inhibitor		IGF-1 × Inhibitor
Inhibitor	F-Ratio	P-Value	F-Ratio	P-Value	F-Ratio	P-Value
H-89	16.95	0.083	0.21	N.S.	25.81	0.016
PD98059	11.84	0.0003	49.81	<0.0001	24.42	<0.0001
LiCl	75.57	<0.0001	3.69	0.071	8.92	0.0008
Go6983	9.09	<0.0001	79.37	<0.0001	6.42	<0.0001
TBCA	53.54	<0.0001	13.55	<0.0001	9.18	0.032

Huh7 human hepatoma cells grown in 96-well micro-cultures were pre-treated with vehicle (control), LiCl, TBCA, Gö6983, H-89, or PD98059 to inhibit GSK-3β, CK2, pan-PKC, PKA, or MEK, and then stimulated for 60 minutes with 10 ng/ml IGF-1. Cellular ELISAs measured immunoreactivity to AAH (A85G6-C-terminus; A85E6 and FB50-N-terminus) and β-actin. Data were analyzed by Two-way ANOVA. Corresponding graphs with post-hoc Fisher LSD results are shown in Figure 6.