Table 3.
Multivariate Cox regression analyses of clinicopathological variables, mRNA and protein expression levels, and breast cancer survival
| Variable | B (SE) | Wald | RR (95 % CI) | P |
|---|---|---|---|---|
| Multivariate survival analysis with TMPRSS1 mRNA expression | ||||
| Nodal status | ||||
| Negative | Ref. | |||
| Positive | 0.786 (0.339) | 5.369 | 2.194 (1.129–4.265) | 0.020 |
| Tumor size | ||||
| T1 | Ref. | |||
| T2 | 0.678 (0.421) | 2.597 | 1.970 (0.864–4.492) | 0.107 |
| T3, T4 | 1.385 (0.504) | 7.563 | 3.997 (1.489–10.729) | 0.006 |
| TMPRSS1 mRNA expression* | ||||
| Low | 0.725 (0.318) | 5.187 | 2.065 (1.106–3.856) | 0.023 |
| High | Ref. | |||
| Multivariate survival analysis with TMPRSS3 mRNA expression | ||||
| Nodal status | ||||
| Negative | Ref. | |||
| Positive | 1.091 (0.320) | 11.596 | 2.976 (1.589–5.575) | 0.001 |
| Tumor size | ||||
| T1 | Ref. | |||
| T2 | 0.317 (0.373) | 0.769 | 1.386 (0.668–2.874) | 0.380 |
| T3, T4 | 1.256 (0.452) | 7.720 | 3.511 (1.448–8.516) | 0.005 |
| TMPRSS3 mRNA expression* | ||||
| Low | 0.745 (0.301) | 6.111 | 2.106 (1.167–3.800) | 0.013 |
| High | Ref. | |||
| Histology | ||||
| Ductal | 0.472 (0.551) | 0.734 | 1.603 (0.545–4.715) | 0.391 |
| Lobular | 1.501 (0.589) | 6.497 | 4.487 (1.415–14.231) | 0.011 |
| Medullary, others | Ref. | |||
| ER status | ||||
| Negative | 0.628 (0.310) | 4.109 | 1.873 (1.021–3.437) | 0.043 |
| Positive | Ref. | |||
| Multivariate survival analysis with combined TMPRSS1-TMPRSS3 mRNA expression | ||||
| Combined mRNA expression† | ||||
| Low | 0.909 (0.325) | 7.803 | 2.482 (1.312–4.698) | 0.005 |
| Others | Ref. | |||
| Nodal status | ||||
| Negative | Ref. | |||
| Positive | 0.975 (0.360) | 7.327 | 2.650 (1.309–5.368) | 0.007 |
| Tumor size | ||||
| T1 | Ref. | |||
| T2 | 0.481 (0.433) | 1.233 | 1.617 (0.692–3.779) | 0.267 |
| T3, T4 | 1.281 (0.505) | 6.427 | 3.600 (1.337–9.691) | 0.011 |
| Multivariate survival analysis with combined hepsin-TMPRSS3 protein expression | ||||
| Nodal status | ||||
| Negative | Ref. | |||
| Positive | 0.905 (0.227) | 15.942 | 2.473 (1.586–3.857) | 0.00007 |
| Tumor size | ||||
| T1 | Ref. | |||
| T2 | 0.176 (0.235) | 0.559 | 1.192 (0.752–1.888) | 0.455 |
| T3, T4 | 0.891 (0.321) | 7.696 | 2.438 (1.299–4.576) | 0.006 |
| Histology | ||||
| Ductal | 0.255 (0.322) | 0.628 | 1.291 (0.687–2.426) | 0.428 |
| Lobular | 0.903 (0.343) | 6.909 | 2.466 (1.258–4.834) | 0.009 |
| Medullary, others | Ref. | |||
| Tumor grade | ||||
| I | Ref. | |||
| II | 0.711 (0.290) | 6.025 | 2.036 (1.154–3.591) | 0.014 |
| III | 0.532 (0.337) | 2.493 | 1.702 (0.880–3.294) | 0.114 |
| Combined protein expression† | ||||
| Low | 0.432 (0.214) | 4.087 | 1.541 (1.013–2.342) | 0.043 |
| Others | Ref. | |||
Note: Analyses included tumor grade, histology, tumor size, nodal status, ER and PR status
B (SE), B coefficient with standard error; HR (95 % CI), hazard ratio of breast cancer death with a 95 % confidence interval; Ref., reference category in the multivariate analysis
*The relative median value of mRNA expression level was used in the analyses
†The ‘combined low group’ included all cases with low expression levels of both genes