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Comparison of COX-1 mRNA in OSE isolated and analyzed from individual follicles F1 (n = 8), F2 (n = 8), F3 (n = 8) (A) and POF-1 (n = 7), POF-2 (n = 6) and POF-3 (n = 6) (B); COX-2 mRNA expression in OSE isolated from individual follicles (C) and POF-1–3 (D). Follicles and POFs were collected immediately after oviposition, RNA was extracted and quantified by real-time PCR. COX-1 and COX-2 mRNA by copy number, normalized to GAPDH. a versus b, P < 0.05; a versus c, P < 0.01 (±SEM)
