COX-1 and COX-2 protein and mRNA expression in the ovary. A and B, COX-1 enzyme mRNA expression was measured using real time PCR while protein levels were analyzed using western blotting in control and flaxseed diet-fed chickens, respectively, (n=6). C and D, COX-2 enzyme mRNA expression was measured using real time PCR while protein levels were analyzed using western blotting in control and flaxseed diet-fed chickens, respectively, (n=6). (0% vs. 15% (P<0.01), 5% vs.15% (P<0.05) and 10% vs. 15% (P<0.05)).