Table 2.
Primers used for real time qPCR
| Gene symbol | Common name | Gene id. | Forward primer | Reverse primer |
|---|---|---|---|---|
| Target | ||||
| PTGS1 | Cyclooxygenase 1 | 427752 | 5’TCAGGTGGTTTCTGGGACATCA3’ | 5’TGTAGCCGTACTGGGAGTT GA3’ |
| PTGS2 | Cyclooxygenase 2 | 396451 | 5’CTGCTCCCTCCCATGTCAGA3’ | 5’CACGTGAAGAATTCCGG TGTT3’ |
| ESR1 | Estrogen Receptor 1 | 396099 | 5’CCACAGCATTCGTGAGAGG3’ | 5’GCATAGTCGTTGCACAC AGC3’ |
| CYP1B1 | Cytochrome P450, family 1, subfamily B, polypeptide 1 | 421466 | 5’CAAGATTCCTGGATGAGAACG3’ | 5’GCTGCACCTTGGA TAATTCC3 |
| CYP1A1 | Cytochrome P450, family 1, subfamily A, polypeptide 1 | 396051 | 5’TGGATACCCTCTGCCTCTCT3’ | 5’GGAACTAAGGGGAAG CGTG3’ |
| CYP3A4 | Cytochrome P450, family 3, subfamily A, polypeptide 4 | 416477 | 5’CCATCTTGGTGAAGGAGTGC3’ | 5’TTCCACTGGTC ATCCATAGC3’ |
| Housekeeping | ||||
| TBP | TATA box binding protein | 395995 | 5’ CGTGAGGGAAATAGGCA 3’ | 5’GACTGGCAGCAAGGAAG 3’ |
| SDHA | Succinate dehydrogenase complex, subunit A | 395758 | 5’ CAGGGATGTAGTGTCTCGT 3’ | 5’GGGAATAGGCTCCTTAGTG 3’ |
| RPL4 | Ribosomal protein L4 | 415551 | 5’ TTATGCCATCTGTTCTGCC 3’ | 5’GCGATTCCTCATCTTACCCT 3’ |