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. 2015 May 27;10(5):e0125221. doi: 10.1371/journal.pone.0125221

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© 2015 Cautain et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 5 — Cell lysates were collected from RCC4-VA and RCC4-VHL cells 24h or 48h after MDN-0066 treatment at 25uM for 24hrs or 48hrs. Blots were probed with antibodies targeting either total β-actin, HIF-1α, PKM2, MCT4, C-Myc, Beclin, LC3b, LDHA or cleaved PARP.-, no treatment, + MDN-0066. In Fig 5b we quantified the intensity of the western blot for each bands density using the LI-COR Aerius software.