Table 6. Results of ABO7 qPCR and Internal Positive Control Test for PCR inhibition.
| Sample | Extract | Concentration (ng/μL) | Variance in % Methylation | Mean Cq Cycle | % Difference (IPC and Sample C q ) |
|---|---|---|---|---|---|
| B170 | 1 | 0.0326 | 3.33 | 24.67 | 1.90 |
| B50 | 1 | 0.0265 | 3.33 | 24.53 | 1.32 |
| C34-1 | 1 | 0.0025 | 84.50 | 28.11 | 16.11 |
| C40-59 | 1 | 0.0013 | 4.25 | 24.91 | 2.89 |
| E10.2 | 2 | 0.0052 | 8.00 | 25.25 | 4.30 |
| E30.A | 2 | 0.0072 | 37.58 | 24.44 | 0.95 |
| E5.1 | 2 | 0.0092 | 72.00 | 26.71 | 10.33 |
| E8.5 | 2 | 0.0293 | 0.50 | 24.64 | 1.78 |
| OH98 | 1 | 0.0140 | 10.67 | 25.12 | 3.76 |
| RIC6 | 1 | 0.0017 | 40.5 | 25.48 | 5.25 |
| Y2.2 | 2 | 0.0184 | 5.67 | 24.82 | 2.52 |
| Y2.3 | 2 | 0.0007 | 32.33 | 24.49 | 1.16 |
| Y2.5 | 2 | 0.0118 | 32.00 | 24.8 | 2.44 |
| Y2.7 | 1 | 0.0050 | 40.50 | 24.84 | 2.60 |
| Y3.4 | 1 | 0.0315 | 5.67 | 24.55 | 1.40 |
| Y3.9 | 2 | 0.0012 | 8.33 | 25.08 | 3.59 |
| IPC Standard | NA | NA | NA | 24.21 | NA |
NA: Not applicable; High levels of inhibition are in bold.