Table 6. Results of ABO7 qPCR and Internal Positive Control Test for PCR inhibition.

Sample	Extract	Concentration (ng/μL)	Variance in % Methylation	Mean Cq Cycle	% Difference (IPC and Sample C q )
B170	1	0.0326	3.33	24.67	1.90
B50	1	0.0265	3.33	24.53	1.32
C34-1	1	0.0025	84.50	28.11	16.11
C40-59	1	0.0013	4.25	24.91	2.89
E10.2	2	0.0052	8.00	25.25	4.30
E30.A	2	0.0072	37.58	24.44	0.95
E5.1	2	0.0092	72.00	26.71	10.33
E8.5	2	0.0293	0.50	24.64	1.78
OH98	1	0.0140	10.67	25.12	3.76
RIC6	1	0.0017	40.5	25.48	5.25
Y2.2	2	0.0184	5.67	24.82	2.52
Y2.3	2	0.0007	32.33	24.49	1.16
Y2.5	2	0.0118	32.00	24.8	2.44
Y2.7	1	0.0050	40.50	24.84	2.60
Y3.4	1	0.0315	5.67	24.55	1.40
Y3.9	2	0.0012	8.33	25.08	3.59
IPC Standard	NA	NA	NA	24.21	NA

NA: Not applicable; High levels of inhibition are in bold.