| DNA | 30 μL |
| Pfx buffer | 10 μL |
| Illumina genomic primers (1.1 & 2.1) | 1 μL each |
| dNTPs (10mM) | 3 μL |
| MgSO4 (50mM) | 2 μL |
| Water | 53 μL |
| Pfx | 1 μL |
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