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. 2014 Dec 15;5(6):135. doi: 10.1186/scrt525

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© Xiang et al.; licensee BioMed Central. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Dental follicle stem/progenitor cell differentiation upon Wnt5a protein exposure. (A, B) Dental follicle stem/progenitor cells showed virtually no alizarin red area upon exposure to Dulbecco’s modified Eagle’s medium (DMEM) without osteogenic supplements for the tested 14 days. (C, D) Dental follicle stem/progenitor cells exposed to osteogenic medium (OM) yielded a moderate alizarin red area. (E, F) Dental follicle stem/progenitor cells upon exposure to both Wnt5a protein (300 ng/ml) and osteogenic medium generated some alizarin red area. (G) Quantitatively, dental follicle stem/progenitor cells upon exposure to Wnt5a protein and osteogenic medium showed significantly greater alizarin red (AR) area than Wnt5a exposure alone, which in turn was more significant than wild-type dental follicle cells (DFC; n = 3; **P <0.01). (H) Real-time quantitative PCR (Taqman) revealed alkaline phosphatase (ALP), osteocalcin (Ocn), Runx2 and Col1a1 mRNA expression upon Wnt5a protein exposure for 7 days (n = 3; **P <0.01). (I) ALP, Ocn and Runx2 protein expression as treated with Wnt5a protein for 7 days. (J, K) Osteoprotegerin (OPG) and receptor activator for nuclear factor-κB ligand (RANKL) mRNA expression when dental follicle stem/progenitor cells were exposed to Wnt5a protein for 3 and 7 days. GAPDH, glyceraldehyde-3-phosphate dehydrogenase; NS, not significant; ***(P <0.01).