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. 2014 Dec 15;5(6):135. doi: 10.1186/scrt525

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© Xiang et al.; licensee BioMed Central. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Wnt5a signaling in dental follicle stem/progenitor cells. (A) Western blot was used to detect phosphorylation-Jun N-terminal kinase (P-Jnk) 1/2 expression of Wnt5a transfected cells and vector control; higher expression of P-Jnk1/2 was observed in Wnt5a transfected cells. (B) Exposure to SP600125 for 3 days, a Jnk inhibitor, attenuated Col1a1, Runx2 and osteocalcin (Ocn) in Wnt5a transfected dental follicle cells relative to vector controls as demonstrated by western blot. (C, D, E, F) Real-time quantitative PCR (Taqman) revealed synergistic effects of Wnt5a when exposed to 100 ng/ml BMP2 for 3 days by upregulating Ocn, Runx2 and Col1a1 mRNA, but not alkaline phosphatase (ALP) activity (n = 3; **P <0.01; ***P <0.001). GAPDH, glyceraldehyde-3-phosphate dehydrogenase; NS, not significant.