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. 2015 May 27;10(5):e0126871. doi: 10.1371/journal.pone.0126871

Fig 1. Initial screening of WALK11 peptide isomers.

Fig 1

(A) The amino acid sequences of the WALK11 peptides tested in this study are presented, with their corresponding serial numbers (WALK11.1-WALK11.11), labeled according to the position of a single tryptophan (W; indicated in bold) in each sequence. (B) Cytotoxicity was evaluated by incubating RAW264.7 cells with each peptide (8–0.5 μM, two-fold serial dilutions) for 24 h, and the cell viability was assessed with an MTT assay as the percentage of surviving cells compared to that of the control cells. (C) The NO inhibitory effects of individual peptides were examined in cells pre-treated with each peptide (2 μM) for 1 h and stimulated by LPS (100 ng/ml) for 24 h. The culture supernatants were harvested and assayed for concentrations of nitrite, represented by bars with the mean ± SEM of independent experiments performed in triplicate.