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. 2015 May 16;16:11. doi: 10.1186/s12867-015-0034-8

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© Jiang et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Expression of miR-19a in HUVECs either transduced with Ad-miR19a and transfected with miR-19a inhibitor. (A) and (B), Quantitative real time PCR revealed that the expression levels of miR-19a were increased after Ad-miR-19a transduction (A), but suppressed by miR-19a inhibitor in a dose-dependent manner (B). Significance is indicated as *P < 0.05. (C) and (D), MiR-19a regulates ASK1 expression. HUVECs were transduced with Ad-miR-19a or Ad-LacZ (50MOI) for 24 h, and then treated with 100 ng/mL LPS for another 24 h. The expression of ASK1 was determined by quantitative real time PCR (C) and western blot (D).