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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Matrix Biol. 2015 Feb 3;43:97–108. doi: 10.1016/j.matbio.2015.01.021

Figure 1.

Figure 1

Proteins from A431 cell extracts bind to fibulin-1. A431 cell extract was passed over fibulin-1 sepharose column. Eight-molar urea was used to elute the fractions. Fractions were run on 4–12% Tris Glycine SDS-PAGE gel then stained with Coomassie blue. The three major bands were sent for sequencing at the MUSC Mass Spectroscopy Facility and identified as MYH9 (200 Kda), fibulin-1 (75 Kda) and actin (42 Kda). FT, flow-through; STD, molecular weight standards; arrowheads depict major eluted proteins.