Table 1.
The primer sequences for single cell RT-PCR
| Target | Primer | Sequence (5′ to 3′) | Product size (bp) | Genomic size (bp) | Sequence |
|---|---|---|---|---|---|
| β-actin | Forward | TGGCTACAGTTTCACCACCA | 212 | 212 | NM_001172909.1 |
| Reverse | GGAAGGAGGGCTGGAAGA | ||||
| TRPV1 | Forward | CCAACAAGAAGGGGTTCACA | 168 | >1,000 | NM_001172652.1 |
| Reverse | ACAGGTCATAGAGCGAGGAG | ||||
| ASIC1 | Forward | CAGGAAGTGCCAGAAAGAGG | 151 | 286 | XM_00347578 |
| Reverse | CGGATGGTGAGGTAGGATGT | XM_00500647 | |||
| ASIC1a | Forward | GCAGGACACGACATACGAGA | 376 | >1,000 | XM_00347578 |
| Reverse | GCGGAGGCAGGTAGATGAG | ||||
| ASIC1b | Forward | GTGCCCCTTGCTCCTCCT | 240 | >1,000 | XM_00500647 |
| Reverse | ATTGCCTGTCCCACCCTTC | ||||
| ASIC2 | Forward | GGAGTTGGGCTTTGGGGTAG | 382 | >1,000 | XM_003469484 |
| Reverse | CTTGGCTGATGTTTTGCTTGG | ||||
| ASIC2a | Forward | GTTCAAGGGGCAGGAGTGT | 338 | >1,000 | XM_003469484* |
| Reverse | GTGGGGGCAGGTATGTGAG | ||||
| ASIC2b | Forward | GCTGCTCTCCTGCAAATACC | 293 | >1,000 | XM_003469484 |
| Reverse | CTACCCCAAAGCCCAACTC | ||||
| ASIC3 | Forward | CGGATGGGACAGTGCTACA | 260 | 485 | XM_003469663.2 |
| Reverse | TGGCAGGACACAAAAGTCTG | XM_005003488.1 | |||
| OGR1 (GPR68) | Forward | CTACATCAGCGTGGGCTTC | 200 | 200 | XM_005004868.1 |
| Reverse | AGACGAGGTGTTGGTCATTGT | ||||
| G2A (GPR132) | Forward | GTCGTTGTCATCTTCCTGGTC | 232 | 232 | scaffold_111* |
| Reverse | GGTGGAGTCTGGTCACTTCCT | ||||
| GPR4 | Forward | CCATCCTCTACTGCCTGGTC | 155 | 155 | scaffold_80* |
| Reverse | CACGCTGCTTCTCTTGGAA | ||||
| TDAG8 (GPR65) | Forward | GCAAGCAAAGAAGGAAAATGA | 139 | 139 | XM_003472463.1 |
| Reverse | GCACAAGGCAGCAGAGAAA | ||||
| TASK1 (KCNK3) | Forward | GGTACAAGAGCCGCGAGAA | 186 | 186 | XM_003472862.2 |
| Reverse | TGGACACGGAGCTGATGG | ||||
| TASK3 (KCNK9) | Forward | GAAACTCAAGGCGGAAGAGA | 391 | >1,000 | NM_001172977.1 |
| Reverse | GCCCATGCAGGAGAAGAAG | ||||
| TALK1 (KCNK16) | Forward | CCAAGGCAACTCCACCAA | 147 | 987 | scaffold_15* |
| Reverse | CCCACCAGGGCATAGAAG | ||||
| TRPV4 | Forward | ATCATCCTCACCTTCGTGCT | 191 | >1,000 | XM_003477915 |
| Reverse | TTGCCCACAGTCACCATCT | ||||
| PKD2L1 | Forward | GGCAAATCCGAACAGTGAAG | 294 | 986 | XM_003479572.2 |
| Reverse | CCAGGAACTCAAAGTCAGCA |
*
The sequences of guinea pig G2A, GPR4 and TALK1 were deduced from the CavPor3 assembly of the guinea pig genome (Feb. 2008 draft, UCSC Genome Bioinformatics Group) by using human, rat and/or mouse adenosine receptor sequences and compared with database sequences if available.