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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Nat Neurosci. 2015 Apr 27;18(6):863–871. doi: 10.1038/nn.4011

Figure 2. aBNST and LHMC4R neurons are not a downstream target for ARCAgRP-driven hunger.

Figure 2

a–b, CRACM from ARCAgRP neurons (red) onto putative post-synaptic aBNSTMC4R (a) and LHMC4R (b) neurons (green) demonstrating the absence of monosynaptic inhibitory connections. c, In vivo optogenetic occlusion schematic involving concomitant stimulation of MC4R soma and ARCAgRP terminals in the aBNST and LH. d–e, ARCAgRP→aBNST (d; n=5) and →LH (e; n=6) ChR2-driven light-cycle food intake was unaffected by simultaneous activation of aBNSTMC4R (Repeated measures ANOVA, main effect of treatment (F(1,4)=1645, p<0.0001), no main effect of genotype (F(1,4)=2.132, p=0.22) and interaction (F(1,4)=0.38, p=0.58; post-hoc: ARCAgRP→aBNST (pre vs stim), ***p=0.0001; ARCAgRP→aBNSTMC4R (pre vs stim), ***p=0.0001) or LHMC4R (Repeated measures ANOVA, main effect of treatment (F(1,5)=359.9, p<0.0001), no main effect of genotype (F(1,5)=0.43, p=0.58) and interaction (F(1,5)=0.16, p=0.70); post-hoc: ARCAgRP→LH (pre vs stim), ***p=0.0009; ARCAgRP→LHMC4R (pre vs stim), **p=0.0013) soma Abbreviations, f, fornix; aca, anterior commissure anterior part. Scale bar in panel c, 100 μm and relates to all images. All values presented as mean±SEM.