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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: Exp Neurol. 2015 Apr 8;269:120–132. doi: 10.1016/j.expneurol.2015.04.001

Figure 4.

Figure 4

GAD-67 immunostaining in Thy1-ChR2-YFP hippocampal sections in P14 mice. (A) Diagram showing the localization of the GABA interneuron network in the CA3 region of the hippocampus. (B) Panoramic view of CA3 shows interneurons co-expressing YFP and GABAergic marker GAD-67. (C) Magnified view of the region indicated in B to show the YFP-positive cells in CA3 in greater detail. (D–F) Single image channels for GAD-67 (D), YFP (E), and TOTO-3 (F) that comprise the merged image in C. Cells co-expressing GAD-67 (D) and YFP (E) are indicated by arrowheads. The densely clustered pyramidal cells in this region of the hippocampus are shown by the TOTO-3 nuclear stain (F). Scale bar: 20μm.